Analisis Kadar Vitamin C Pada Buah Delima (Punica granatum L.) Merah Dan Putih Secara Spektrofotometri UV-VIS
Abstract
Kandungan dalam buah delima sangat beragam, salah satu diantaranya adalah vitamin C. Vitamin C adalah nutrisi yang penting untuk memelihara kesehatan seperti pada tulang, pembuluh darah, proses peremajaan sel, hingga antioksidan. Vitamin C banyak berasal dari buah-buahan salah satunya buah delima (Punica granatum L.). Penelitian ini bertujuan untuk menganalisis kadar vitamin C dalam buah delima (Punica granatum L.) merah dan putih dengan analisis kualitatif dan kuantitatif. Analisis kualitatif dilakukan dengan menggunakan pereaksi spesifik, yaitu ammonium molybdate, Fehling A dan B yang menunjukkan sampel positif yang mengandung vitamin C, dan vitamin C dianalisis secara kuantitatif menggunakan spektrofotometri UV-Vis pada panjang gelombang 570 nm. Hasil penelitian menunjukkan kadar vitamin C dalam daging buah delima merah 0.24475 mg/g dan daging delima 0.11577 mg /g.
References
2. Sirat DW. Antioksidan dalam Bakso Rumput Laut Merah Eucheuma Cottonii. 2012;1(1):1–4.
3. Andrés AI, Petrón MJ, Adámez JD, López M, Timón M. PT. Meat Sci [Internet]. 2017; Available from: http://dx.doi.org/10.1016/j.meatsci.2017.02.013
4. Natalello A, Luciano G, Morbidini L, Valenti B, Pauselli M, Frutos P, et al. Effect of feeding pomegranate by-product on fatty acid composition of ruminal digesta, liver and muscle in lambs. J Agric Food Chem [Internet]. 2019;acs.jafc.9b00307. Available from: http://pubs.acs.org/doi/10.1021/acs.jafc.9b00307
5. Syed QA, Batool Z, Shukat R, Zahoor T. L UPINE PUBLISHERS Nutritional and Therapeutic Properties of Pomegranate. 2018;1(4):115–20.
6. Gheflati A, Mohammadi M, Jolfaie NR, Heidari Z, Abargouei AS, Nadjarzadeh A. Does pomegranate consumption affect weight and body composition ? A systematic review and meta ‐ analysis of randomized controlled clinical trials. 2019;(January):1–12.
7. Fedarko NS. Theories and Mechanisms of Aging. 2018;19–26.
8. Sharma J, Maity A. Pomegranate Phytochemicals : Nutraceutical and Therapeutic Values. 2010
9. Torregrosa-garc A, Vicente Á, Luque-rubia AJ, Javier FL. of Trained Cyclists after an Exhausting Endurance Trial : A Randomised Controlled Trial. 2019
10. Sudarmadji, S., Haryono, B., dan Suhardi. 1997. Prosedur Analisa Untuk Bahan Makanan dan Pertanian. (Edisi keempat) Liberty. Yogyakarta.
11. Mulja, M., Suharman. 1995. Analisis Instrumental. AirlanggaUniversity Press. Surabaya
12. Selimović A, Salkić M, Selimovic A. Direct spectrophotometric determination of L-ascorbic acid in pharmaceutical preparations using sodium oxalate as a stabilizer. Vol. 11, Int. J. Basic Appl. Sci. 2011.
13. Sudjarwo dan Badriah. 2010. Optimization and Validation of Visible-Spectrofotometric Method For Determination Ascorbic Acid In Jeruk Bali (Citrus maxima) Fruit, Airlangga University
14. Russo M, Cacciola F, Arena K, Mangraviti D, de Gara L, Dugo P, et al. Characterization of the polyphenolic fraction of pomegranate samples by comprehensive two-dimensional liquid chromatography coupled to mass spectrometry detection. Nat Prod Res [Internet]. 2019;0(0):1–7. Available from: https://doi.org/10.1080/14786419.2018.1561690
15. Luo X, Cao D, Li H, Zhao D, Xue H, Niu J, et al. Complementary iTRAQ-based proteomic and RNA sequencing- based transcriptomic analyses reveal a complex network regulating pomegranate ( Punica granatum L .) fruit peel colour. Sci Rep [Internet]. 2018;(October 2017):1–13. Available from: http://dx.doi.org/10.1038/s41598-018-30088-3
16. Saminathan T, Bodunrin A, Singh N V, Devarajan R, Nimmakayala P, Jeff M, et al. Genome-wide identification of microRNAs in pomegranate ( Punica granatum L .) by high-throughput sequencing. BMC Plant Biol [Internet]. 2016;1–16. Available from: http://dx.doi.org/10.1186/s12870-016-0807-3
