Toxicity of Standardized Ethanolic Extracts from Duchesnea indica (Jacks.) Focke Plants Using the BSLT Method

Risda Waris; Selpida  Handayani; Wisdawati; Rahni  Hossani

doi:10.33096/woh.v7i2.1328

Risda Waris Phytochemistry Division-Pharmacognosy Phytochemical Laboratory, Faculty of Pharmacy, Universitas Muslim Indonesia, Makassar
Selpida Handayani Pharmacognosy Division-Pharmacognosy Phytochemical Laboratory, Faculty of Pharmacy, Universitas Muslim Indonesia, Makassar
Wisdawati Pharmacognosy Division-Pharmacognosy Phytochemical Laboratory, Faculty of Pharmacy, Universitas Muslim Indonesia, Makassar
Rahni Hossani Allied Health Science Departement, Walailak University, Nakhon Si Thammarat, Thailand

DOI: https://doi.org/10.33096/woh.v7i2.1328

Keywords: Duchesnea indica (Jacks)Focke, Standardization, Toxicity, Traditional medicine

Abstract

Duchesnea indica (jacks. ) focke., known as a medical plant that is used as raw ingredients for traditional medicines and herbs to increase the immune system because plants have the properties to cure and prevent various diseases. The objective of this study is to examine the quality of the duchesnea indica leaves extract by standardization method of specific and non specific parameters as well as the toxicity assay using using the Brine Shrimp Letality Test (BSLT). The results of specific parameter research include the identity of extracting duchesnea indica, Organoptic tests form extract samples that are used to extract thick, It's brown, Typical smell, astringent taste. The content of water soluble compounds is 18.67%, the content of ethanol soluble compounds is 0.03%, based in phytochemical screening test, the duchesnea indica leaves extract contain phenolic, flavonoid, tannin, Saponin and terpenoid. Non-specific parameters carried out include loss on drying of grounded Duchesne indica leaves at 0.029%, loss on drying of DuchesneA indica leaves extract at 0.026%, specific gravity determination at 0.83%, extract water content determination of 2.15%, total ash content is 3.70%, non-soluble acid of ash content is 1.32%. Thus, from these results, we can conclude that Duchesne indica leaf extract meets quality standards for extracting raw materials that can be used for traditional medicine. Research results: The standardized ethanol extract of duchesnea indica leaves is toxic to Artemia salina leach larvae.

References

DAFTAR PUSTAKA
1. Waris, R., Mursyid A., M., (2021)Aktivitas Antiinflamasi Ekstrak Etanolik Daun Arbenan [Duchesnea indica (Jacks.) Focke]. Jurnal Fitofarmaka Indonesia;8(1)18-22.
2. Yang,W.E ,Ho,. Y.C,., Tang, C. M.,Hsieh, Y. S., Chen, P.N., Lai, C.T.,Yang S. F.,Lin, C.W., (2019). Duchesnea indica extract attenuates oral cancer cells metastatic potential through the inhibition of the matrix metalloproteinase-2 activity by down-regulating the MEK/ERK pathway. Phytomedicine2019 Oct;63:152960. doi: 10.1016/j.phymed.
3. Zhu, M., Dong, X. & Guo, M. (2015) ‘Phenolic Profiling of Duchesnea indica Combining Macroporous Resin Chromatography (MRC) with HPLC-ESI-MS/MS and ESIIT-MS’, Molecules, 20(12), pp. 22463– 22475. doi: 10.3390/molecules201219859.
4. Nuraziza, Seniwaty, Waris, R. (2017), ‘Uji aktivitas antioksidan ekstrak etanol daun arbenan (Duchesnea indica (Andr.) Focke) dengan metode DPPH’, Jurnal As’Syfa Farmasi Universitas Muslim indonesia, Makassar.
5. Departemen Kesehatan RI. (2020). 'Farmakope Indonesia edisi VI'. Departemen Kesehatan Republik Indonesia.
6. Sartinah, A., Yamin., Nurhasanah., Arba, M., Akib, N.I dan Adjeng, A.N.T., (2020), Uji Toksisitas Akut Ektstrak dan Fraksi Kulit Batang Ketapang Laut (Terminalia carappa L.) menggunakan Metode BSLT, Pharmauho: Jurnal Farmasi, Sains dan Kesehatan, Vol.3, no.1, pp. 42-47.
7. Saifudin, A., Rahayu, V., & Teruna, HY., (2011), Standarisasi Obat Alam, Graha ilmu, Yogyakarta.
8. Utami, Y. P., Umar, A. H., Syahruni, R., & Kadullah, I. (2017). 'Standardisasi Simplisia dan Ekstrak Etanol Daun Leilem ( Clerodendrum'. Journal of Pharmaceutical and Medicinal Sciences, 2(1): 32–39.
9. Mus S, Rahimah, S., Taebe B.,, Musrifah, Muslimin, L.(2020). Acute Toxicity Test of Kopasanda (Chromolaena odorata L) Leaves Ethanol Extract Using Brine Shrimp Lethality Test (BSLT) Method. Journal of Pharmaceutical and Medicinal Sciences 2020 5(2): pp 44-47.
10. M I Rasyid, H Yuliani, N Triandita et al. (2020). Toxicity Test LC50 of Pineung Nyen Teusalee Seeds (Areca catechu) Extract by Brine Shrimp Lethality Test (BSLT) Method. IOP Conf. Ser.: Earth Environ. Sci. 515.
11. Fatimah R, Santoso, B.S.A (2020). Toksisitas Akut Dekok Daun Kersen (Muntingia Calabura) Menggunakan Metode Bslt (Brine Shrimp Lethality Test). Pharmacy Medical Journal. Vol.3 No.2.
12. Direktorat Jenderal Pengawasan Obat dan Makanan. (2000). Parameter Standar Umum Ekstrak Tumbuhan Obat. Jakarta : Direktorat Jenderal Pengawasan Obat dan Makanan.
13. Hui, J. X. Qiang, G.J. Wen, S.C. & Liu, X.M. (2012) ‘High frequency shoot organogenesis in juvenile leaf of Duchesnea indica (Andr.) Focke’, African Journal Of Biotechnology, 11(17), pp. 4039–4044. doi: 10.5897/AJB11.3116.
14. Menteri Kesehatan RI. (2009). Farmakope Herbal Indonesia Edisi I : standar untuk simplisia dan ekstrak yang berasal dari tumbuhan atau tanaman obat.
15. Najib, A. (2018) Ekstraksi Senyawa Bahan Alam. Yogyakarta: Deepublish.
16. Parwata, I. M. O. A., (2017), Buku Ajar Obat Tradisional. Jurusan Kimia Laboratorium Kimia Organik Fakultas Matematika Dan Ilmu Pengetahuan Alam Universitas Udayana.